DNA Sequencing refers
different methods and technologies, which are used for determining the sequence
or order of the nucleotide bases-adenine, guanine, cytosine, and thymine in a
DNA molecule. The adverse use of advanced DNA sequencing i.e next generation
sequencing has extremely accelerated biological research and discovery day by
day.
DNA Sequencing
was conducted through extensively laborious and rigorous methods before 1970,
but the journey of advance DNA sequencing started with Maxam Gilbert Sequencing.
The Chain-termination methods, Dye-terminator sequencing, High-throughput
sequencing, Lynx Therapeutics' Massively Parallel Signature Sequencing (MPSS),
Polony Sequencing have come into the limelight for developing the technology.
454 pyrosequencing, Illumina (Solexa) sequencing, SOLiD sequencing, DNA
nanoball sequencing etc. have started the next generation sequencing using different
types of innovative technology.
The advent of
next generation Sequencers are contributing a huge amount of data for analysis.
However, it has increased the complexities of genomic sequencing workflows but
enriched biological discoveries to the greater extent. A tightly integrated,
scalable, high-performance computing platform with intelligent data management
systems and uncompromised security could make all the difference in advancing DNA
Sequencing. Moreover, transparency of the huge data generated by the sequencers
must be decoded by the trustworthy platform.
Today’s Bioinformatics
moving towards technology advancement and leveraging cloud computing to answer
the parallel processing of gamut of data securely. Cloud computing
infrastructures expected to be more scalable and transparent to make usability
more conducive to Bioinformatics data scientists.
